Alkaline Phosphatase (ALP) Activity Assay Kit

Sizes: 96T, 500 Assays

Catalogue Numbers: E-BC-K091-M-96, E-BC-K091-M-500

Citations, Manuals and MSDS Available upon request.

Target Synonym: ALP

Research Areas: Liver and Renal Function

Result Type: Enzyme Activity

Detection Instrument: Microplate reader

Tested Sample Types: Serum, plasma, tissue, cells

Assay Time: 30 min

Detection Type: Colorimetric method

Sensitivity: 0.13 King unit/100 mL

Detection Range: 0.13-50 King unit/100 mL

Test Principle: Alkaline phosphatase decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.

Inter CV: 8.5

Intra CV: 5.1

Storage: This product can be stored at 2~8°C for 12 months with shading light.

Shipping: Ice bag