BRD5631 is an autophagy enhancer, enhances autophagy through an mTOR-independent pathway. BRD5631 affects several cellular disease phenotypes previously linked to autophagy, including protein aggregation, cell survival, bacterial replication, and inflammatory cytokine production.

Product information

CAS Number: 2446154-91-0

Molecular Weight: 501.62

Formula: C30H35N3O4

Chemical Name: N-[(3S,6S,7S)-7-methoxy-3,6,9-trimethyl-10-oxo-3,4,5,6,7,8,9,10-octahydro-2H-1,4,9-benzoxadiazacyclododecin-12-yl]-[1,1'-biphenyl]-4-carboxamide

Smiles: CO[C@@H]1CN(C)C(=O)C2=CC(=CC=C2OC[C@H](C)NC[C@@H]1C)NC(=O)C1C=CC(=CC=1)C1C=CC=CC=1

InChiKey: XGMSCYLPQZMHLL-YHGPEZAFSA-N

InChi: InChI=1S/C30H35N3O4/c1-20-17-31-21(2)19-37-27-15-14-25(16-26(27)30(35)33(3)18-28(20)36-4)32-29(34)24-12-10-23(11-13-24)22-8-6-5-7-9-22/h5-16,20-21,28,31H,17-19H2,1-4H3,(H,32,34)/t20-,21-,28+/m0/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

BRD5631 (10 μM, 48 hours) leads to increased levels of LC3-II in HeLa cells. BRD5631 stimulates formation of new autophagosomes, which is consistent with its ability to increase numbers of autolysosomes in the mCherry-GFP-LC3 assay. BRD5631 does not enhance autophagy by directly inhibiting mTOR or the mTOR signaling pathway. BRD5631 suppressed IL-1β secretion in an autophagy-dependent manner.

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